Detection of Parasitized Rice Weevils in Wheat Kernels with Near-Infrared Spectroscopy

w L r w h c w h i a i d m t n ( e S t s u ( t r p r Individual kernels of wheat containing immature ice weevils, Sitophilus oryzae (L.) (Coleoptera: Curcuionidae), parasitized by Anisopteromalus calandrae Howard) (Hymenoptera: Pteromalidae) could be sepaated from uninfested kernels and kernels that conained unparasitized weevils by using near-infrared NIR) spectroscopy. Wheat kernels from cultures of the arasitoid were X-rayed and sorted into uninfested ernels, kernels infested with weevil larvae or pupae, ernels containing parasitoid larvae feeding on host eevils, and kernels containing parasitoid pupae and emains of the host. Kernels were automatically posiioned and scanned (400–1700 nm) with the NIR sysem. Kernels containing parasitoid pupae were comletely differentiated from kernels containing weevil upae as well as all other kernels in this automated ystem. With the appropriate integration of NIR specroscopy and seed-sorting instrumentation, kernels ontaining parasitoid pupae could be automatically ollected from cultures of A. calandrae during the ass rearing of this beneficial insect.